the effect of am281, a cannabinoid antagonist, on memory performance during spontaneous morphine withdrawal in mice

نویسندگان

g vaseghi

m rabbani

v hajhashemi

چکیده

abrupt cessation of morphine leads to withdrawal signs and cognitive deficits. endocannabinoid system is activated during withdrawal; therefore, the aim of the present study was to assess the effects of am281, cannabinoid antagonist/inverse agonist, on memory deficit following spontaneous morphine withdrawal. cognition was evaluated by using the object recognition task. the novel object recognition task was tested in a square wooden open-field box using objects. the test was consisting of three sections: 15 min exploration, first trial for 12 min and second one for 5 min. in the second trial the difference in exploration between a previously seen object and a novel one, was considered as an index of memory performance (recognition index - ri). male mice were made dependent by increasing doses of morphine (30-90 mg/kg) subcutaneously twice daily for 3 days. am281 (0.62, 1.25 and 2.5 mg/kg) were used in chronic form concurrent with morphine i.p. or acutely (2.5, 5 and 10 mg/kg) on the last day. ri was evaluated on the third day 4 h after the last dose of morphine. chronic administration of am281 at 2.5 mg/kg improved ri to the 22.1 ± 4.8 and single dose of am281 at 5 mg/kg improved the memory impairment to the 8.5 ± 4, as compared with vehicle-treated which was 4.8 ± 2.5. the results suggested that administration of am281 at a dose of 2.5 mg/kg in chronic form and 5 mg/kg in acute dose improved memory.

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منابع مشابه

The effect of AM281, a cannabinoid antagonist, on memory performance during spontaneous morphine withdrawal in mice

Abrupt cessation of morphine leads to withdrawal signs and cognitive deficits. Endocannabinoid system is activated during withdrawal; therefore, the aim of the present study was to assess the effects of AM281, cannabinoid antagonist/inverse agonist, on memory deficit following spontaneous morphine withdrawal. Cognition was evaluated by using the object recognition task. The novel object recogni...

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عنوان ژورنال:
research in pharmaceutical sciences

جلد ۸، شماره ۱، صفحات ۵۹-۰

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